1x running buffer (Thermo Fisher)
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1x running buffer
1x Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+transfer+buffer/Semi+dry+blot+transfer+buffer/pmc13176446-111-3-6
Average 95 stars, based on 1 article reviews
1x Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+transfer+buffer/Semi+dry+blot+transfer+buffer/pmc13176446-111-3-6
Average 95 stars, based on 1 article reviews
1x running buffer - by Bioz Stars,
2026-10
95/100 stars
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Membrane:Article Title: Transcription factor cooperativity at a GATA3 tandem DNA sequence determines oncogenic enhancer-mediated activation. Article Snippet: Samples were separated on the NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific) for 1 h at 170 V in 1x MOPS buffer (Thermo Fisher Scientific). .. Proteins were transferred onto a PVDF membrane (Bio-Rad) in Article Title: A step-by-step guide to performing cancer metabolism research using custom-made media. Article Snippet: .. Cell lysates were then mixed with 5× Laemmli buffer containing 10% glycerol (Sigma-Aldrich), 1% β-mercaptoethanol (SigmaAldrich), 1.7% SDS (Carl Roth), 62.5 mM Trizma base (pH 6.8; Sigma-Aldrich), and bromophenol blue (Sigma-Aldrich), followed by heating at 95°C for 5 min. Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) using self-cast 8%, 10%, or 12% acrylamide (Carl Roth) gels in a Mini-PROTEAN Tetra system (Bio-Rad) with running buffer (0.2 M glycine [Sigma-Aldrich], 25 mM Trizma base [Sigma-Aldrich], 0.1% SDS [Sigma-Aldrich]) at 80–120 V. Subsequently, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) at 45 V for 2 h in Article Title: TIR signaling activates caspase-like immunity in bacteria Article Snippet: Samples were incubated at 95°C for 3 min and 10 μL were separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) in 1x MOPS buffer (Thermo Scientific). .. Gels were transferred to a nitrocellulose membrane (Invitrogen #LC2001) for 1 hour at 20 V in Article Title: Alternative Import-Channels And Destinations Of Mitochondrial PINK1 Controlled By Trans-Membrane-Domain Structural Plasticity Article Snippet: Protein samples to be analysed by western blotting were prepared first for SDS-PAGE by boiling gel samples at 95°C in 1xLDS + 25mM DTT prior to running on a 4-12% BOLT gel (Thermo Fisher Scientific), run at 200V for 25min. .. Gels were transferred onto 0.45um nitrocellulose blotting membrane (Cytiva) in Article Title: A step-by-step guide to performing cancer metabolism research using custom-made media Article Snippet: .. Cell lysates were then mixed with 5× Laemmli buffer containing 10% glycerol (Sigma-Aldrich), 1% β-mercaptoethanol (Sigma-Aldrich), 1.7% SDS (Carl Roth), 62.5 mM Trizma base (pH 6.8; Sigma-Aldrich), and bromophenol blue (Sigma-Aldrich), followed by heating at 95°C for 5 min. Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) using self-cast 8%, 10%, or 12% acrylamide (Carl Roth) gels in a Mini-PROTEAN Tetra system (Bio-Rad) with running buffer (0.2 M glycine [Sigma-Aldrich], 25 mM Trizma base [Sigma-Aldrich], 0.1% SDS [Sigma-Aldrich]) at 80–120 V. Subsequently, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) at 45 V for 2 h in Article Title: Heat shock induces alternative polyadenylation through dynamic DNA methylation and chromatin looping. Article Snippet: 10 μg of cell lysate per sample were resolved on SDSPAGE in NuPAGE reducing sample buffer (Thermo Fisher Scientific #NP0001) using the Novex system at 120V for 1 hour. .. Proteins were transferred to PVDF membrane using a Polyacrylamide Gel Electrophoresis:Article Title: A step-by-step guide to performing cancer metabolism research using custom-made media. Article Snippet: .. Cell lysates were then mixed with 5× Laemmli buffer containing 10% glycerol (Sigma-Aldrich), 1% β-mercaptoethanol (SigmaAldrich), 1.7% SDS (Carl Roth), 62.5 mM Trizma base (pH 6.8; Sigma-Aldrich), and bromophenol blue (Sigma-Aldrich), followed by heating at 95°C for 5 min. Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) using self-cast 8%, 10%, or 12% acrylamide (Carl Roth) gels in a Mini-PROTEAN Tetra system (Bio-Rad) with running buffer (0.2 M glycine [Sigma-Aldrich], 25 mM Trizma base [Sigma-Aldrich], 0.1% SDS [Sigma-Aldrich]) at 80–120 V. Subsequently, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) at 45 V for 2 h in Article Title: A step-by-step guide to performing cancer metabolism research using custom-made media Article Snippet: .. Cell lysates were then mixed with 5× Laemmli buffer containing 10% glycerol (Sigma-Aldrich), 1% β-mercaptoethanol (Sigma-Aldrich), 1.7% SDS (Carl Roth), 62.5 mM Trizma base (pH 6.8; Sigma-Aldrich), and bromophenol blue (Sigma-Aldrich), followed by heating at 95°C for 5 min. Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) using self-cast 8%, 10%, or 12% acrylamide (Carl Roth) gels in a Mini-PROTEAN Tetra system (Bio-Rad) with running buffer (0.2 M glycine [Sigma-Aldrich], 25 mM Trizma base [Sigma-Aldrich], 0.1% SDS [Sigma-Aldrich]) at 80–120 V. Subsequently, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) at 45 V for 2 h in Western Blot:Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in Fluorescence:Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in Electrophoresis:Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in Article Title: Using circular RNAs to target toxic RNA-binding proteins in amyotrophic lateral sclerosis Article Snippet: .. Subsequently, proteins were separated on Novex WedgeWell 8–16% Tris-Glycine Gels (Invitrogen) in 1x running buffer (25 mM Tris-base, 192 mM glycine, 0.1% SDS) and transferred to either 0.45 μm Amersham Hybond polyvinylidene difluoride (PVDF) blotting membranes (Cytiva) for chemiluminescence western blotting or 0.45 μm Immobilon-FL PVDF Transfer Membranes (Millipore) for fluorescence western blotting, both in |